equation between light intensity and sugar concentration

For the treatment, draw up a small amount ~1 mL of detergent solution, and then draw the baking soda solution up to ~3-5 mL.Push the plunger almost to the bottom but don't crush the disks.Separate the two parts of the syringe, drop 10 of the spinach disks inside, reassemble the syringe.Use the metal hole punch to cut out 20 circular disks from the fresh spinach leaves, 10 for a control and 10 for a treatment.Light source (fluorescent is good because it produces light without much heat).

Cup 2: Baking soda solution (treatment).

3 clear plastic cups or beakers (250 mL to 500 mL).

Liquid dish soap solution (dissolve 5 mL in 250 mL of water).

Baking soda solution (dissolve some baking soda powder in water).

10 mL or larger plastic syringe (without needle) - get one from your local pharmacy.

The carbonic acid then breaks down into water and carbon dioxide gas, which is why dissolving baking soda in water causes it to fizz. When we dissolve baking soda (NaHCO 3) in water, carbonic acid (H 2CO 3) and sodium hydroxide (NaOH) are formed. Finally, we could experimentally vary the light intensity to demonstrate the effect of light intensity on the process. We can also demonstrate that the bubbles only form in the presence of strong light, by moving the experiment into the dark and making further observations. While we cannot prove in this experiment that the bubbles are oxygen without a gas probe, we can demonstrate, by use of a control, that the bubbles only form when the leaves are submerged in a sodium bicarbonate solution (which releases CO 2) and not when they are submerged in pure water.

The light-independent reaction or Calvin Cycle takes carbon dioxide and fixes it in three-carbon molecules which will eventually be synthesized into glucose.Įxperiment: We will conduct a simple experiment using spinach leaves to demonstrate that, in the presence of light and carbon dioxide, leaf tissues produce gas bubbles.

The light reaction is used to convert sunlight into chemical energy stored in ATP and another energy storage molecule called NADP.

The energy accumulated in Stage 1 is used to drive Stage 2. Stage 2 can work in the light or in the dark. Oxygen molecules are colored to show their fate.

The basic reaction is:Ħ C O 2 + 12 H 2 O + e -> 2 C 6H 12 O 6 + 6 O 2Ĭarbon dioxide + water + light energy -> glucose + oxygen Organisms that can make their own food are called autotrophs, and are at the base of the food chain. With water, light energy from the sun, and carbon dioxide from the air, photosynthetic organisms are able to build simple sugars. Photosynthesis is one of the most important anabolic chemical reactions that allows life to exist on Earth.

Equation between light intensity and sugar concentration